agarose beads (Vector Laboratories)
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Vector Laboratories
agarose beads
Agarose Beads, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/AL-1023S/Agarose+bound+succinylated+Wheat+Germ+Agglutinin+(WGA)/pmc13050799-293-11-13
Average 93 stars, based on 67 article reviews
Agarose Beads, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/AL-1023S/Agarose+bound+succinylated+Wheat+Germ+Agglutinin+(WGA)/pmc13050799-293-11-13
Average 93 stars, based on 67 article reviews
agarose beads - by Bioz Stars,
2026-10
93/100 stars
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Pull Down Assay:Article Title: Time-resolved multiomics profiling reveals chromatin O-GlcNAc modification promotes senescence-associated transcriptional program. Article Snippet: The antibodies and beads used for IP were anti-JUN (Abcam, #ab40766, 1:50), anti-GATAD2A (CST, #17705, 1:50), anti-O-GlcNAc CTD110.6 (CST, #9875, 1:1000), anti-O-GlcNAc EPR19847 (Abcam, #ab202665, 1:50), antiFlag-magnetic beads (Bimake, # B26102), and anti-HA-magnetic beads (Bimake, #B26202). .. For lectin pull down assay, each cell lysate was incubated with sWGA bound Article Title: Time-resolved multiomics profiling reveals chromatin O-GlcNAc modification promotes senescence-associated transcriptional program Article Snippet: The antibodies and beads used for IP were anti-JUN (Abcam, #ab40766, 1:50), anti-GATAD2A (CST, #17705, 1:50), anti-O-GlcNAc CTD110.6 (CST, #9875, 1:1000), anti-O-GlcNAc EPR19847 (Abcam, #ab202665, 1:50), anti-Flag-magnetic beads (Bimake, # B26102 ), and anti-HA-magnetic beads (Bimake, # B26202 ). .. For lectin pull down assay, each cell lysate was incubated with sWGA bound Incubation:Article Title: Time-resolved multiomics profiling reveals chromatin O-GlcNAc modification promotes senescence-associated transcriptional program. Article Snippet: The antibodies and beads used for IP were anti-JUN (Abcam, #ab40766, 1:50), anti-GATAD2A (CST, #17705, 1:50), anti-O-GlcNAc CTD110.6 (CST, #9875, 1:1000), anti-O-GlcNAc EPR19847 (Abcam, #ab202665, 1:50), antiFlag-magnetic beads (Bimake, # B26102), and anti-HA-magnetic beads (Bimake, #B26202). .. For lectin pull down assay, each cell lysate was incubated with sWGA bound Article Title: O-GlcNAcylation of DDB1 at Ser-764 by OGT promotes cancer cell stemness in colorectal cancer through increased polyubiquitination of p53. Article Snippet: Protein concentrations of the cell lysates were measured using the Bio-Rad AR TIC LE IN PR ES S protein assay (Hercules, CA, USA). .. The lysates were incubated overnight at 4°C with agarose-conjugated Article Title: The crosstalk between ubiquitination and GlcNAcylation of CHAF1A regulates HIV-1 latency and reactivation. Article Snippet: The supernatants were collec ted, and protein concentrations were determined using the BCA assay (Thermo Fisher Scientific). .. For each sample, 250 μg total protein was incubated overnight at 4°C with 50 Month XXXX Volume 0 Issue 0 10.1128/jvi.01518-2517 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/j vi o n 06 D ec em be r 20 25 b y 2a 09 :b ac 5: 3a 21 :2 72 3: :3 e6 :f . μL of pre-washed succinylated Article Title: OGT mediates O-GlcNAcylation of MEIS2 and affects palatal osteogenic development. Article Snippet: Succinylated wheat germ agglutinin pull-down assays For succinylated wheat germ agglutinin (sWGA) pull down assays, each lysate underwent denaturing in glycoprotein denaturing buffer and digestion with Peptide N-Glycosidase (New England Biolabs, P0704S) for the removal of N-linked glycoproteins. .. After being centrifuged, the supernatants underwent 2-h incubation with sWGA bound Article Title: Time-resolved multiomics profiling reveals chromatin O-GlcNAc modification promotes senescence-associated transcriptional program Article Snippet: The antibodies and beads used for IP were anti-JUN (Abcam, #ab40766, 1:50), anti-GATAD2A (CST, #17705, 1:50), anti-O-GlcNAc CTD110.6 (CST, #9875, 1:1000), anti-O-GlcNAc EPR19847 (Abcam, #ab202665, 1:50), anti-Flag-magnetic beads (Bimake, # B26102 ), and anti-HA-magnetic beads (Bimake, # B26202 ). .. For lectin pull down assay, each cell lysate was incubated with sWGA bound Article Title: O- GlcNAcylation of DDB1 at Ser-764 by OGT promotes cancer cell stemness in colorectal cancer through increased polyubiquitination of p53 Article Snippet: Protein concentrations of the cell lysates were measured using the Bio-Rad protein assay (Hercules, CA, USA). .. The lysates were incubated overnight at 4 °C with agarose-conjugated Article Title: OGT mediates O-GlcNAcylation of MEIS2 and affects palatal osteogenic development Article Snippet: For succinylated wheat germ agglutinin (sWGA) pull down assays, each lysate underwent denaturing in glycoprotein denaturing buffer and digestion with Peptide N-Glycosidase (New England Biolabs, P0704S) for the removal of N-linked glycoproteins. .. After being centrifuged, the supernatants underwent 2-h incubation with sWGA bound Magnetic Beads:Article Title: POM121 O-GlcNAcylation facilitates bone metastasis in non-small cell lung cancer through enhanced c-MYC nuclear import and ECM reprogramming Article Snippet: .. Protein A/G magnetic beads (Thermo, cat#88802), primary antibody (TABLE S ; validated for IP), species-matched IgG antibody (TABLE S ), or Lysis:Article Title: POM121 O-GlcNAcylation facilitates bone metastasis in non-small cell lung cancer through enhanced c-MYC nuclear import and ECM reprogramming Article Snippet: .. Protein A/G magnetic beads (Thermo, cat#88802), primary antibody (TABLE S ; validated for IP), species-matched IgG antibody (TABLE S ), or |
![A Protein levels of OGT, OGA and O-GlcNAc in bone-metastatic H460-BM subline and parental H460 cells. Blots are representative of three independent experiments. B Schematic of pulldown strategy to identify O-GlcNAc-modified proteins contributing to enhanced bone metastasis in H460-BM cells. C Left, significantly enriched ( p < 0.05) biological processes (GO analysis) for O-GlcNAc-modified proteins in H460-BM cells (top 10 pathways shown). Right, Enrichment of O-GlcNAcylation nucleoporins identified by mass spectrometry. D O-GlcNAcylation protein levels of POM121 in bone-metastatic H460-BM subline and parental H460 cells. O-GlcNAcylated proteins were pulled down using <t>sWGA-beads.</t> Blots are representative of three independent experiments. E H460-BM cells expressing Myc (tag)-POM121 were treated with or without OGA inhibitor Thiamet G (TMG, 10 mM, 24 h). GlcNAc (+) indicates that sWGA-beads were pre-blocked with GlcNAc (500 mM, 24 h) prior to pulldown. Immunoprecipitation (IP) and immunoblots (IB) were performed using the indicated antibodies. Data are representative of three independent experiments. F H460-BM cells expressing Myc (tag)-POM121 and mutations were subjected to sWGA-beads pulldown or anti-Myc (tag)-IP. Immunoblots identified Ser199 as an O-GlcNAc modification site. Data are representative of three independent experiments. G Mapping the O-GlcNAcylated residue Ser199 on POM121 by liquid chromatography-tandem mass spectrometry (LC-MS/MS). H , I O-GlcNAcylation of POM121 at the Ser199 site in NSCLC promotes bone metastasis in nude mouse model. H Bioluminescence imaging of nude mice four weeks after intracardiac injection of H460-BM or H460-BM POM121-S199A cells (1 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\times$$\end{document} × 10 5 cells/mouse). Data are represented as mean ± SEM; n = 6 mice/group, *** p < 0.001, unpaired t-test. I Representative H&E staining and tumor area quantifications of bone metastasis sections from mice in ( H ). Dashed lines demarcate tumor boundaries. Scale bar, 20 µm. Data are represented as mean ± SEM; **** p < 0.0001, unpaired t-test.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5952/pmc12885952/pmc12885952__41388_2026_3687_Fig1_HTML.jpg)
